Which procedure is not required when the shotgun approach to sequencing is modified as sequencing by synthesis, in which many small fragments are sequenced simultaneously?

Which procedure is not required when the shotgun approach to sequencing is modified as sequencing by synthesis, in which many small fragments are sequenced simultaneously? 






A) use of restriction enzymes
B) sequencing each fragment
C) cloning each fragment into a plasmid
D) ordering the sequences
E) PCR amplification







Answer: C


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